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| Seminar of Visiting Professor Hassan Y. Aboul-Enein |
Professor Hassan Y. Aboul-Enein
Pharmaceutical and Medicinal Chemistry Department, National Research Centre, Dokki,
Reversed-phase high performance liquid chromatography (HPLC) is one of the most utilized forms of chromatography. The C8 and C18 stationary phases are the most widely used for reversed-phase HPLC. However, analysts occasionally encounter difficult separations for which selectivity, ruggedness or reproducibility are not obtained easily using traditional C8 and C18 phases. These separations might require the use of novel or selective phases that interact with analytes in a manner different than C8 or C18 phases .
¢ These types of stationary phases separate compounds based upon selective stationary phase interactions such as steric recognition charge transfer or π–π interactions. One class of selective or novel phases that have been used for a variety of separations are fluorinated phases
¢ Fluorinated phases can provide different elution orders, leading to enhanced selectivity for difficult-to-separate compounds
¢ Fluorinated phases also offer the possibility to use simpler mobile phases and avoid using extreme pH conditions and complex mobile-phase preparations.
¢ Fluorinated packings also exhibit excellent selectivity for compounds having hydroxyl, nitro and other polar groups in large ring systems.
This presentation will present several types of fluorinated stationary phases and show several examples of how this extra selectivity has been used to achieve chromatographic separation for a broad range of applications.
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